Composite

Part:BBa_K1357009:Experience

Designed by: Matthew Tucker   Group: iGEM14_Virginia   (2014-10-09)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1357009

Firstly, we got BBa_K1357009 from the 2017 DNA Distribution Kit Plates and transformed it to the E.Coli DH5α. After a one-week incubation, a big bacterial colony grown on the plate.

XMU-TEST_T1.jpeg

Secondly, we extracted plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid BBa_J61002 with the promoter J23100, then transformed into E.coli DH5α again. We observed the strong blue just one day later. The result shows that BBa_K1357009 works well.

XMU-TEST_T3.jpeg

XMU-TEST_T2.jpeg

User Reviews

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